Muto T, et al. - Researchers conducted the study for comparing the impacts of 30-gauge vs 32-gauge needles on vitreous reflux (VR) frequency and intraocular pressure (IOP) following first-time intravitreal aflibercept injections. Overall, 116 individuals (116 eyes) who received intravitreal injections using 30-gauge needles and 104 people (104 eyes) who received the same injection using 32-gauge needles have been reviewed. The medical records of 116 individuals each receiving an intravitreal injection using a 30-gauge needle (median age: 67.5 ± 13.9 years) and 104 individuals each receiving the same injection with a 32-gauge needle (median age: 66. 3 ± 10.6 years) were compared from January 2015 to June 2019. There was no significant difference in VR frequency between patients who were injected using 30-gauge needles and patients who were injected using 32-gauge needles. Findings suggested no association of VR frequency with needle size, lens status, or axial length. Immediately after intravitreal injection, patients receiving injections using 30-gauge needles had higher IOP.