Li W, et al. – This study is conducted to report a new strategy of performing preimplantation genetic diagnosis (PGD) in screening (target–) mutation–free embryos. The first multiple annealing and looping–based amplification cycles (MALBAC)–PGD attempt in autosomal dominant polycystic kidney disease (ADPKD) patient resulted in a healthy live birth free of ADPKD and chromosomal anomalies. MALBAC–PGD also enables selecting embryos without aneuploidy together and target gene mutation, thereby increasing implantation and live birth rates.