Lang HB, et al. - Given the involvement of transient receptor potential canonical (TRPC) channels in neovascularization repairing after vascular injury in many tissues, researchers here examined the role of TRPC1, 3, and 6 as well as the involved mechanism in human retina vascular endothelial cells (HREC) under high glucose (HG) conditions. Under HG, hyper osmosis, and normal conditions, the culture of HRECs in vitro was performed. Using RT-polymerase chain reaction, Western blot, and cell immunohistochemistry, they determined the expression of TRPC1, 3, and 6 in the cells at 24 and 48 h. In various concentrations, SKF96365 acted on HG cultured HRECs, detection of the expression of vascular endothelial growth factor (VEGF) was done using the same methods above; and cell proliferation, migration, and lumen formation were assessed using the CCK-8, Transwell, cell scratch assay, and Matrigel assay. Outcomes revealed that expression of TRPC1 and 6 could be increased in HRECs in presence of HG. Inhibition of the TRPC pathway led to a decrease in VEGF expression as well as prevention of proliferation, migration, and lumen formation of HRECs induced by HG. The findings suggest a possible utility of inhibition of TRPC channels as a drug target for diabetic retinopathy.