Nam GH, et al. - In this study, researchers utilized natural ingredients, Trapa japonica fruit was a hard, aquatic plant that grows in ponds or marshes and contains protein and starch in order to assess the anti-aging and health-promoting benefits of bioactive substances. It was found that the role of the fermented hot-water trapa japonica fruit extract to separate the active ingredients with antiwrinkle effects in vitro and ex vivo situation for human dermal fibroblast cell proliferation by activating TGF-β1/GSK-3β/β-catenin pathway.