Ikemoto Y, et al. - Researchers assessed the prevalence of multiple pregnancy with zygotic splitting after single embryo transfer (SET) via performing a retrospective observational study, based on 937,848 SET cycles in a Japanese ART registry system between 2007 and 2014. Outcomes revealed that the prevalence of multiple pregnancy with zygotic splitting after SET was 1.36%. As per the statistical analysis, compared with singleton pregnancies, zygotic splitting pregnancies were associated with frozen-warmed ET cycles, blastocyst culture, or AH. The authors suggested that health-care providers should consider whether to counsel couples about the increase in the risk of zygotic splitting associated with some embryo manipulations.