de Vilbiss EA, et al. - Researchers examined how pre-conceptional biomarkers of maternal folate status (folate and homocysteine) are associated with reproductive outcomes in folate-replete women. From the Effects of Aspirin in Gestation and Reproduction (EAGeR) trial which was a block-randomized, double-blind, placebo-controlled trial whereby women were randomized to daily low-dose aspirin (81 mg/day) or placebo and all women received folic acid (400 μg/day), they analyzed a cohort of 1,228 women who reported 1-2 previous pregnancy losses and no documented infertility from 4 clinical sites in the US (2006-12) and were attempting pregnancy for up to 6 menstrual cycles. Among women with two prior losses, a non-linear association was observed between higher plasma homocysteine and higher risks of pregnancy loss. They identified no significant relationships between serum folate and any reproductive outcome or between plasma homocysteine and anovulation or conception.