Evans V, Behr M, Gangwar A, et al. - Chronic myofascial pain syndrome (MPS), the most common cause of chronic musculoskeletal pain, often presents with elevated muscle stiffness, and the myofascial trigger point (MTrP). Via performing this review, researchers sought for evidence in literature for the use of imaging in the role of classifying and explaining the physiology of MTrPs. As there are only a few imaging techniques that have been carried out on MTrPs, the imaging techniques are determined to be able to effectively image complex skeletal muscle microstructure. As part of a scoping review, a systematic search was performed from three medical databases (CINAHL, EMBASE and MEDLINE) from year to year to determine past MTrP imaging, as well as assessing imaging techniques conducted on the microstructure of muscle. In previous studies, differentiation of active, latent MTrPs involved use of ultrasound, however, their underlying anatomical structure was not adequately addressed in these studies. The standard method of imaging skeletal muscle remains MRI. As per the existing MRI literature, the diffusion tensor imaging (DTI) technique can aid in determining muscle injury, strain, and structure. DTI is suggested to be the most effective imaging modality that has been employed to define the microstructure of muscle and hence, it could be optimal to image the MTrP. Theoretically, techniques like high angular resolution diffusion imaging (HARDI) and diffusion kurtosis imaging (DKI) have potential for analysis of muscle, which may yield more detailed information representative of finer muscle structural features.