Brotons P, Saucedo J, Simó S, et al. - The diagnostic performance of the cobas Liat Cdiff test, a novel single-step automated polymerase chain reaction (PCR) assay, for rapid diagnosis of toxigenic Clostridioides difficile infection (CDI) in stool samples from children with clinical symptoms of CDI was determined along with time of analytical process and agreement of results with those of a 2-step diagnostic algorithm. The sequential algorithm incorporated an enzyme immunoassay (EIA) targeting antigen glutamate dehydrogenase (GDH), enterotoxin-A and cytotoxin-B, and a confirmatory PCR in EIA GDH-positive and toxin-negative samples. They collected a total of 122 specimens from 91 children (mean age, 8 years; 69.7% male). cobas Liat Cdiff led to identification of 24 (19.7%) positive samples. Per findings, detection yield obtained using cobas Liat Cdiff was similar to that of the 2-step diagnostic algorithm while the time of the analytical process was markedly reduced in stool samples from children suspected of CDI.