Chen W, et al. - Researchers tested a hypothesis that a large hydrodynamic radius (R_h) of secondary calciprotein particles (CPP2) and/or a fast speed of transformation (T₅₀) are related to vascular calcification (VC) in participants with chronic kidney disease (CKD) Stages 4–5 (22 without VC and 23 with VC) and healthy volunteers (HV). To test this hypothesis, they developed a high throughput, microplate-based assay using dynamic light scattering (DLS) to measure the transformation of primary calciprotein particles (CPP1) to CPP2, R_h of CPP1 and CPP2, T₅₀ and aggregation of CPP2. A Kauppila score >6 or an Adragao score ≥3 defined VC. They found an independent association of R_h of CPP2, but not T₅₀, with VC in patients with CKD Stages 4–5.