González-Saldivar G, et al. - In the present study, the researchers sought to test depth of field (DOF) and lateral resolution (LR) on digitally assisted vitreoretinal surgery (DAVS) with different camera aperture values and TV viewing distances in order to determine maximal optical performance. Five retinal surgeons objectively tested DOF and LR at low (10×), medium (15×), and high (20×) magnification on a conventional microscope (CM) and DAVS. On DAVS, DOF increased significantly as camera aperture size was reduced and as microscope magnification was reduced. The authors' study is the first to test different aperture, magnification, and TV distance settings, and DAVS can significantly outperform a CM with respect to DOF and LR, if optimized. Optimized visual performance for DAVS is achieved with a camera aperture of 30% and a TV viewing distance of no more than 1.5 m. Maximal microscope magnification to use LR during macular surgery and low-to-medium magnification to increase DOF for overall vitrectomy surgery is suggested.