Setti AS, de Almeida Ferreira Braga DP, Provenza RR, et al. - Via performing a historical cohort study, researchers examined how sperm DNA fragmentation (SDF) affects clinical outcomes of assisted reproductive technology in women with different age ranges. Among 540 couples undergoing intracytoplasmic sperm injection cycles, three groups were formed by splitting cycles according to maternal age: ≤ 36 years old (n = 285), 37–40 years old (n = 147), and > 40 years old (n = 108). The Sperm Chromatin Dispersion test was used to evaluate semen samples for SDF. For younger patients (≤ 36 years old) and those between 37 and 40 years of age, they identified no significant disparities in laboratory and clinical outcomes for cycles with < 30% SDF or ≥ 30% SDF. However, injection of older oocytes with sperm derived from samples with high SDF index, led to development of embryos of poor quality that consequently resulted in lower implantation and pregnancy rates and higher miscarriage rates, in intracytoplasmic sperm injection cycles from women with advanced maternal age.