Al-Rawaf HA, et al. - Researchers investigated the diagnostic value of a set of microRNAs (miRNAs) for pain intensity in adolescents with chronic fatigue syndrome (CFS). The correlation of the expression of these miRNAs with the levels of inflammatory markers and pain-related comorbidities was then determined in adolescents with CFS and healthy controls (HCs). Participants comprised a total of 150 adolescents (12 to 18 years of age): 100 adolescents with CFS and 50 adolescents as HCs. Adolescents with CFS vs comparable nonfatigued HCs had significantly higher pain thresholds. In CFS patients, pain least affected the ability to enjoy life and relations with others. In patients with CFS, differential expression of miR-558, miR-146a, miR-150, miR-124, and miR-143 was significantly downregulated and notably interfered with pain intensity and frequency; there was a direct association of the differential expression with higher expression of the immune inflammatory-related genes tumor necrosis factor-α, interleukin-6, and cyclooxygenase-2 in adolescents with CFS.