Pujol A, et al. - Researchers investigated if time to ICSI affects reproductive outcomes. In vitro aging of mature oocytes was observed to have a significant negative effect on the chances to become pregnant. Reproductive results, for patients using their own oocytes and with a transfer of fresh embryos, are expected to improve with limiting incubation time of mature oocytes in the embryology laboratory. Findings thereby suggest that biochemical and clinical pregnancy diminish progressively as time between oocyte pick up (OPU) and ICSI increases after fresh embryo transfer.