Mobarrez F, et al. - By analyzing blood samples from healthy young volunteers who were asked to perform brief active e-cigarette inhalations, researchers determined if e-cigarettes with and without nicotine induce distinct vascular responses in this randomized, double-blind, crossover study. This analysis involved 17 healthy occasional smokers who inhaled 30 puffs of e-cigarette vapor during 30 min. They measured extracellular vesicles (EVs) of endothelial and platelet origin in order to ascertain vascular alterations. A raised level of circulating EVs of endothelial and platelet origin was detected as a consequence of as few as 30 puffs of nicotine-containing e-cigarette vapor, this may imply underlying vascular alterations. A rise in platelet EVs expressing CD40 ligand was brought about by e-cigarette vapor without nicotine, but nicotine, as an ingredient in the vapor, appeared to have a more compelling influence on extracellular vesicle formation and protein composition.