Lyu X, et al. – A real–time quantitative reverse transcription PCR (qRT–PCR)–based multi–fusion gene screening method was elucidated in this study, with the capacity to determine 22 fusion genes commonly found in leukemia. The utility of a fluorescent qRT–PCR (F–qRT–PCR)–based multi–fusion gene screening method was brought to light as an efficient one–step diagnostic procedure. It served as an effective alternative to lengthy conventional diagnostic procedures requiring both cytogenetic analysis, followed by targeted quantitative reverse transcription (qRT–PCR) methods. This facilitated timely management of the patient.