Buell JF, Helm J, Mckillop IH, et al. - As complex abdominal wall reconstruction employing biologic mesh can result in elevated recurrence rates, nonincorporation, and high perioperative costs, a novel decellularization method was developed and applied to porcine muscle fascia to mirror target-tissue architecture. Researchers here analyzed mechanical strength and tissue-graft incorporation. Subcutaneous implantation of the muscle-fascia mesh was done in rats ( n = 4/group) and the cohorts were killed 1 to 4 weeks later. Histological or immunohistochemical examination of explants was done. Mechanical testing revealed strength equivalent to that of a commercially available biological mesh (AlloDerm), with mechanical strength attributable to the fascia component. This study affirms that a DNA-free composite abdominal wall (muscle-fascia) scaffold can be successfully developed via a decellularization process and can be implanted intraspecies without rejection. Widening this approach may aid in exploitation of the angiogenic capacities of decellularized muscle, concomitant with the inherent strength of decellularized fascia, to conduct preclinical analyses of graft strength in animal models in vivo.