Association between fungal contamination and eye bankâprepared endothelial keratoplasty tissue: Temperature-dependent risk factors and antifungal supplementation of optisolâgentamicin and streptomycin
JAMA Ophthalmology Nov 05, 2017
Brothers KM, et al. - Researchers ascertained if eyebank-processed endothelial keratoplasty tissue was at a greater risk of contamination than unprocessed tissue and they intended to model eyebank processing with regard to room temperature exposure on Candida growth in optisolÂgentamicin and streptomycin (GS) with and without antifungal supplementation. The presence of detectable Candida growth in donor rim cultures, related to a higher rate of post-keratoplasty infection, was discovered in endothelial keratoplasty tissue vs other uses at the time of transplantation. This could partly be attributed to the eyebank preparation processes extending the time of tissue warming. A decrease was noted in the growth of Candida species in optisol-GS through reduced room temperature incubation and the addition of antifungal agents. In order to reduce the risk of infection, these data warranted additional exploration.
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