Sahin G, et al. - This study on 152 eyes recruited from Erzurum Region Training and Research Hospital, Turkey, was undertaken to determine differences in retinal nerve fiber layer thickness between ambylopic and fellow eyes and ambylopic and control eyes. In ambylopic group, fellow eyes, and control eyes, the detected temporal retinal nerve fiber layer thickness (RNFL-T) was 77.27 ± 10.38 μ, 79.31 ± 9.53 μ, and 81.46 ± 9.86 μ, respectively, and superior RNFL-T was 136.23 ± 18.52 μ, 131.91 ± 13.80 μ, and 135.56 ± 14.94 μ respectively; nasal RNFL-T was 87.99 ± 13.05 μ, 82.16 ± 12.33 μ, and 85.50 ± 10.62 μ respectively. In the same order, 144.85 ± 18.39 μ, 140.55 ± 16.92 μ, and 143.47 ± 17.75 μ were the respective values for inferior RNFL-T in these groups. No relation was shown between the presence of amblyopia and RNFL-T, therefore, anisometropic amblyopia could be ignored in patients with disease that could be identified and followed via RNFL thickness.