Ozer K, et al. - Researchers reported the distribution of platelets in the plasma after centrifugation steps. Findings suggested a significant difference in the blood analysis including MPV and platelet concentrations between the layers. A total of 87% of the entire platelet mass was contained in the buffy coat and lower third of plasma layers. Platelets should not be argued only on their concentration, rather the estimation of platelets with their yielded concentration and volume with MPV together while comparing PRP preparation protocols and kits was considered appropriate.