Altman MC, et al. - Researchers intended to determine networks of immune responses that occur in children who develop allergy and asthma, using RNA (ribonucleic acid) sequencing of peripheral blood mononuclear cells (PBMCs) collected at age 2. PBMCs from the cases showed higher levels of expression of natural killer (NK) cell related genes. After cockroach (CR) or dust mite (DM) allergen but not tetanus antigen stimulation, differential expression of 244 genes was seen in PBMCs from the cases vs the control group. This study offers important mechanistic insight into an early life immune pathway involved in T helper 2 polarization leading to development of allergic asthma.