Twin papers lend clues into how the brain organizes itself, offering new avenues for studying psychiatric disorders.
Columbia scientists have identified a gene that allows neurons that release serotonin to evenly spread their branches throughout the brain. Without this gene, these neuronal branches become entangled, leading to haphazard distribution of serotonin, and signs of depression in mice. These observations shed light on how precise neuronal wiring is critical to overall brain health, while also revealing a promising new area of focus for studying psychiatric disorders associated with serotonin imbalance – such as depression, bipolar disorder, schizophrenia and autism.

The findings were published in the journal Science.

This research is the result of parallel efforts by Dr. Maniatis, his lab and collaborators across Columbia to understand how individual neurons in the brain ‘see’ each other — and how each of their hundreds, or even thousands, of branches wind through the brain without getting tangled up along the way.

To investigate this problem, Dr. Maniatis and his team focused on a group of genes called clustered protocadherins, or Pcdhs. More than a decade ago, Dr. Maniatis’ lab discovered the human Pcdh gene cluster, and later studies by Maniatis and others revealed that these genes encode a cell surface “barcode” by which individual neurons can distinguish themselves from other neurons. Subsequently, collaborative studies with fellow Zuckerman Institute structural biologists Barry Honig, PhD, and Larry Shapiro, PhD, revealed the precise mechanism by which the Pcdh code is assembled at the cell surface, and how this code is “read” when neurons come in contact with each other. This allows neurons to prevent entanglements by recognizing – and steering clear of – their own branches, a process known as self–avoidance.

In the two papers published in the journal Science, Dr. Maniatis and his team examined the function of Pcdhs in the wiring of olfactory sensory neurons, and serotonergic neurons. The olfactory sensory neuron (OSN) study revealed that the diversity of Pcdhs, working together, produced the necessary combinations of cell–coating molecules to provide each neuron its unique identity. In the absence of diversity, OSNs fail to wire properly in the brain, and the mice fail to distinguish between different odors.

The serotonergic neuron study revealed another important function of Pcdhs. “The main job of these neurons is to distribute serotonin uniformly throughout the brain, which is responsible for maintaining mood balance,” said Dr. Maniatis, who is also director of Columbia’s Precision Medicine Initiative. “To do this, the neurons lay their branches out in a precise, evenly–spaced pattern — a process called axonal tiling. However, the exact mechanism that allows them to do this remained elusive.”

In a series of experiments in mice, Dr. Maniatis’ team pinpointed a single gene within the Pcdh cluster, called Pcdhac2, that was responsible for the ability of serotonergic neurons to assemble into a tiled pattern throughout the brain, and thus evenly distribute serotonin.

“We were surprised to find that, unlike other neurons that displays distinct barcodes of diverse Pcdhs, all serotonergic neurons display a single functional recognition protein,” said Dr. Maniatis. “Thus, serotonergic axonal branches can recognize and repel one another, leading to their even spacing.”

“We found that deleting the Pcdhac2 caused serotonergic neuron branches to become tangled and clumped together,” Dr. Maniatis continued. “Serotonin was released, but it wasn’t distributed evenly throughout the brain.”

Silencing Pcdhac2 also resulted in striking behavioral changes. Compared to normal, healthy mice, Pcdhac2–deficient mice showed behavioral despair and enhanced fear memory.