Researchers at CeMM and the Medical University of Vienna presented a preliminary report on the clinical impact of an integrated ex vivo approach termed pharmacoscopy. The procedures measure single-cell drug responses of millions of individual cells to hundreds of possible treatments in small biopsies from cancer patients.

The interims analysis of the first-ever clinical trial with the approach, published in the current issue of the journal The Lancet Haematology, highlights the potential of the method: 88.2% of patients receiving pharmacoscopy-guided treatment achieved partial or complete remission, compared to 23.5% to their own previous treatment. Further, the median progression-free survival increased four-fold. Retrospectively, pharmacoscopy also predicted the response of AML patients to first-line treatment with 90% accuracy. These results show that pharmacoscopy can assist decision-making of the responsible clinicians effectively and thus represent a powerful tool for practical precise and personalized medicine.

Patients suffering from refractory and relapsed blood cancers often have few treatment options and short survival times. At this stage, identifying effective therapies can be challenging for doctors. Even state-of-the-art genetic analyses, due to the high heterogeneity of cancer cells and the impact of the various mutations on their drug response, do often not suffice to instruct personalized treatments. Pharmacoscopy, a technology developed by scientists at the CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences and tested for its clinical efficacy by clinicians of the Medical University of Vienna, offers a functional approach: hundreds of drug options can be quickly pre-tested ex vivo in small liquid biopsy samples collected from individual patients.

The effects of each drug on the individual cells are quantified using high-throughput and highcontent automated confocal microscopy. In combination with specially developed analysis methods and machine learning and other unique algorithms, pharmacoscopy allows quantification of never-before visualized phenotypes. The method was first presented last April in the journal Nature Chemical Biology.

The multidisciplinary team publishing the current study is spearheaded by Giulio Superti-Furga, scientific director of CeMM and professor at the Center for Physiology and Pharmacology of Medical University of Vienna together with professor Philip Staber, program director for Lymphoma, Chronic Lymphatic Leukemia, and T-cell Lymphoma of the Medical University of Vienna.

While the clinical study is still recruiting, interim analysis of the program showed that 88.2% of the patients recruited (15 out of 17) who received pharmacoscopy-monitored personalized therapies achieved partial or complete remission, while only 23.5% (4 out of 17) responded similarly well to their previous respective treatments.

In addition, the median progression-free survival of patients who were treated in accordance to pharmacoscopy-guided therapy increased from 5.7 week to 22.6 weeks compared to their last line of treatment. Further, in a retrospective study organized to specifically determine the ability of the method to stratify responding and non-responding newly-diagnosed patients with acute myeloid leukemia (AML), resulted in 90% accuracy. Before, such accuracy in prediction of treatment outcome was unachievable, with or without genetic assays.

“Having a robust, fast, and reliable predictive test at our disposal during the patient treatment process, especially at the time of relapse where a new intervention must be selected quickly, will change how medical doctors prioritize drugs to use for late-stage patients,” said Philipp Staber, principal investigator of the clinical trial.

“Evidence that the pharmacoscopy approach is helpful for clinical evaluation of therapy is wonderful. Single-cell functional analysis of primary material gives unprecedented resolution and precision that we are sure to further develop in the future to address yet more diseases,” added Giulio Superti-Furga, whose goal at the beginning of activities of the Research Center for Molecular Medicine ten years ago was to create scientific advancements able to positively impact medical practice.

The article is titled, “Image-based ex-vivo drug screening for patients with aggressive haematological malignancies: Interim results from a single-arm, open-label, pilot study.”