Glioma stem cells are responsible for glioblastoma’s tumorigenesis, treatment resistance and subsequent tumor recurrence.

A collaborative team of neuro–oncology surgeon/scientists — led by Ichiro Nakano, MD, PhD, University of Alabama at Birmingham, and Maode Wang, MD, Xi’an Jiaotong University, Xi’an, China – has discovered a unique and previously unidentified molecular mechanism that maintains glioma stem cells, and they have tested it as a potential therapeutic target in glioblastoma, using a novel small molecule inhibitor they designed and synthesized.

This international study was launched when they found that another inhibitor for advanced cancers, called OTS167, had a negative outcome in a clinical trial.

They found that a different molecular target, NEK2, evolved after OTS167 treatment for glioblastoma, and they used computer–based drug design to target NEK2. The resulting NEK2 inhibitor, called CMP3a, was able to inhibit growth in pre–clinical models of glioblastoma, both in culture and in mouse brains. When combined with radiation, CMP3a has a synergistic effect to attenuate growth of glioblastoma cells in culture.

“We are currently in the process of pharmacokinetic and pharmacodynamic analyses with CMP3a to design an early–phase clinical trial for glioblastoma and other NEK2/EZH2–dependent cancers,” they said in an article published in The Journal of Clinical Investigation.

Through a series of detailed experiments, the researchers revealed how NEK2 promotes tumor growth and resistance – they found that NEK2 protein binds to EZH2, an oncogenic histone H3 methyltranferase, and this binding protects EZH2 from protein degradation in the glioma stem cells. EZH2 was already known to regulate the self–renewal and survival of glioma stem cells. Thus, by stabilizing EZH2, NEK2 promotes tumor propagation.

In a clinical study of 44 patient glioblastoma brain tissues, Nakano, Wang and colleagues found that NEK2 expression was closely associated with EZH2 expression. NEK2 expression also correlated with poor prognosis for the patients, and NEK2 was substantially elevated in recurrent tumors after therapeutic failure. An independent validation cohort included 56 patient samples.

NEK2, they concluded, appears to play a key role in maintaining glioma stem cells by stabilizing the EZH2 protein, and the small molecule inhibitor CMP3a is a potential novel therapeutic agent for glioblastoma.

The researchers had previously studied the MELK–EZH2–STAT3 signaling axis as a central regulator for glioma stem cells in glioblastoma. A MELK inhibitor showed short–term efficacy against human glioma xenografts in a mouse model, but the cultures soon became resistant to the MELK inhibition. Unexpectedly, those resistant glioma cultures retained their EZH2 dependence, prompting the researchers to search how the EZH2 levels were maintained.

Experiments with short hairpin RNAs to knock down NEK2 expression showed that NEK2 is required for glioma stem cell propagation in culture and for glioma tumor growth in the animal model. The researchers found that NEK2 formed a protein complex with EZH2 to phosphorylate and protect EZH2 from proteasome–dependent degradation in glioma stem cells. They also showed that NEK2 promoted radiation resistance in glioma stem cells.

The researchers next designed their novel, clinically applicable, small molecule inhibitor, CMP3a. CMP3a selectively inhibits NEK2 kinase activity in glioma stem cells. Glioma sphere cells showed high sensitivity to this lead candidate for glioblastoma therapy, and normal human astrocytes were markedly resistant to CMP3a. When CMP3a was tested against a screen of 97 kinases, representing all kinase clusters, only three kinases showed more than 65 percent inhibition, indicating that CMP3a is relatively selective for NEK2 inhibition.