Egeland NG, et al. - Researchers sought ascertain the prognostic value of Myristoylated alanine-rich C kinase substrate like-1 (MARCKSL1) protein expression for distant metastasis-free survival (DMFS) in a new cohort of lymph-node negative (LN^-) breast cancer patients. Evaluation of MARCKSL1 expression was performed using immunohistochemistry in 151 operable T_1,2N₀M₀ LN^- breast cancer patients. Outcomes do not confirm the value of MARCKSL1 expression as a prognostic factor in this cohort. Changes in diagnostic and treatment guidelines between the discovery and validation cohorts seem to provide a possible explanation to the finding.