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Ultra-thin DSAEK using an innovative artificial anterior chamber pressuriser: A proof-of-concept study

Graefe's Archive for Clinical and Experimental Ophthalmology Apr 30, 2021

Ruzza A, Parekh M, Avoni L, et al. - In this retrospective laboratory and clinical study, researchers reported the impact of establishing and maintaining a high intracameral pressure (ICP) of 200 mmHg on UT-DSAEK graft preparation utilizing an artificial anterior chamber pressuriser (ACP) control unit (Moria SA, Antony, France). Using an ACP system, four paired donor corneas were mounted on an artificial anterior chamber and subjected to 70 mmHg (“low”) and 200 mmHg (“high”) ICP. The central corneal thinning rate was measured after 5 min utilizing AS-OCT and the endothelial cell viability was analyzed employing trypan blue and live/dead staining following 70 mmHg and 200 mmHg ICP. ACP control unit for UT-DSAEK graft preparation aids in consistently obtaining UT-DSAEK grafts without compromising endothelial cell viability.

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