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Two-tier approach combining molecular and culture-based techniques for optimized detection of vancomycin-resistant enterococci

Diagnostic Microbiology and Infectious Disease Aug 31, 2017

Both A, et al. – Researchers performed this work to compare a two–tier approach consisting of PCR–based screening and cultural confirmation of positive results to the conventional approach solely based on culture on selective media for optimized detection of vancomycin–resistant enterococci. The introduction of a molecular assay for rapid detection of vancomycin resistant enterococci (VRE) from rectal swabs combined with cultural confirmation proved reliable and time saving, especially in a setting of low VRE prevalence and predominance of vanA positive strains.
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