Gebremicael G, Alemayehu M, Sileshi M, et al. - From a total of 262 study participants with five clinical groups ie, 57 TB patients coinfected with HIV (HIV+TB+), 87 active TB Patients (TB cases), 71 HIV infected without active and latent TB infection (HIV+TST-), 22 latent TB infection (TST+) and 25 healthy controls (TST-), micronutrients concentration level was measured. Between active TB and latent (LTBI) or healthy controls, there was a notable variation in the serum concentration levels of iron, vitamin A and vitamin B₁₂. Six months following treatment, the serum concentration levels of vitamin A, vitamin B₁₂ and iron in tuberculosis became indifferentiable from the levels of LTBIs and healthy control people. At the end of TB treatment, the concentration levels of iron and vitamin B₁₂ in HIV+TB+patients were normalized to the levels noted in healthy controls (TST–) irrespective of HAART treatment. Nevertheless, in HIV+TB+patients HAART untreated at the end of TB treatment, the vitamin A concentration level was not normalized to the levels recognized in healthy controls (TST–) or HAART untreated HIV+TST–. Thus, detecting serum concentration levels of vitamin B₁₂ and vitamin A could be used as a biomarker of the diagnostic method of active TB despite HIV-infected individuals. Furthermore, in TB-HIV-co-infected individuals, identifying the serum concentration of vitamin B₁₂ might also be used for TB treatment responses monitoring biomarkers irrespective of HAART (in)eligibility and therapy.