Argaman Y, Kisler LB, Granovsky Y, et al. - In view of the relation of altered pain modulation and resting-state functional connectivity (rsFC) with migraine pathology and clinical manifestation, researchers here investigated the correlation of pain modulation psychophysical measures with resting-state networks and rsFC between bottom-up and top-down pain modulation areas. Temporal summation of pain (TSOP) and conditioned pain modulation (CPM) tests were performed in 32 episodic migraineurs and 23 age-matched healthy individuals, these were followed by a resting-state imaging scan. Healthy individuals exhibited correlation of more efficient CPM with 1) stronger rsFCs of the posterior cingulate cortex, with the ventromedial prefrontal cortex and with the pregenual anterior cingulate cortex; 2) weaker rsFC of the anterior insula with the angular gyrus. On the other hand, migraineurs exhibited alteration of the association between CPM and rsFC. Outcomes imply that the functional connectivity within the default mode network (DMN) elements and the functional coupling between the DMN and pain inhibitory brain areas is associated with pain inhibition efficiency. Despite alteration in migraineurs, this interplay allows normal pain inhibition. Findings thereby indicate a possible functional adaptation of the DMN and its role in pain inhibition in health and migraine.