Crone M, et al. - In this study, researchers investigated how black cohosh (BC) alone and combined with hormones and antihormones, influence cell viability and expression of estrogen receptor alpha (ER-α), progesterone receptor (PR), and BRCA1 in both T-47D and MCF-7 cell lines. They cultured cells in charcoal-stripped serum prior to their treatment and subsequent protein extraction. After 24-hour treatment with 80-500 μM BC, significant changes in ER-α, PR, and BRCA1 protein levels were observed in Western blot analysis. Outcomes revealed the considerable anti-cancer characteristics of black cohosh. This study may notably enhance the knowledge regarding the molecular effects of BC on ER-α, PR, and BRCA1 in breast cancer cells.