He H, Bissonnette R, Wu J, et al. - This study was designed to develop a global transcriptome of tape strips from lesional as well as nonlesional skin of adults suffering from moderate-to-severe atopic dermatitis (AD) and psoriasis. Experts collected 20 tape strips from lesional and nonlesional skin of patients with AD and psoriasis and skin from controls (n = 20 each). RNA sequencing (RNA-seq) was performed on the strips. It was noted that AD and psoriasis tissues shared rises in levels of dendritic cell and T-cell markers (CD3, ITGAX/CD11c, and CD83), but preferential T_H2 skewing was demonstrated by AD tissues, whereas higher levels of expression of T _H17-related, T _H1-related, and innate immunity–related products characterized psoriasis. Overall, distinct immune as well as barrier signatures in lesional and nonlesional AD and psoriasis skin were revealed via RNA-seq tape strip profiling, indicating their usefulness as a minimally invasive alternative to biopsies for identifying disease biomarkers.