den Dekker BM, et al. - Through a prospective single-center observational cohort study that involved 120 women referred for stereotactic vacuum-assisted biopsy of 129 mammographically suspicious lesions between December 2017 and October 2018, researchers ascertained the minimum number of stereotactic 9-gauge vacuum-assisted biopsy specimens needed to build a final histopathological biopsy diagnosis of mammographically suspicious breast lesions. A final histopathological diagnosis was reached following six specimens, in 95% of the procedures. Following nine specimens, the final biopsy diagnosis was authenticated in all 131 cases. In the subgroup of 41 patients with ductal carcinoma in situ (DCIS) or invasive diagnosis at biopsy, there were eight procedures where calcifications were retrieved prior to getting the diagnostic specimen. In six out of 30 excised lesions classified as DCIS and in one out of four excised high-risk lesions, the underestimation of following resection diagnosis occurred. In conclusion, with six stereotactic 9-gauge vacuum-assisted biopsy specimens, in 95% (95%CI 90%-98%) of the biopsy procedures, a final histopathological biopsy diagnosis could be authenticated. Taking nine 9-gauge specimens appears to be optimal. Ending the stereotactic vacuum-assisted breast biopsy procedure as soon as calcifications are retrieved may lead to false-negative results.