Hiwasa T, et al. - This study sought to evaluate whether serum anti-DIDO1, anti-CPSF2, and anti-forkhead box J2 (FOXJ2) antibodies as predictive risk markers for acute ischemic stroke. Researchers used serological identification by cDNA expression cDNA libraries and the protein array method for the screening of antigens recognized by serum IgG antibodies in patients with atherosclerosis. They used recombinant proteins or synthetic peptides derived from candidate antigens as antigens to compare serum IgG levels between healthy donors and patients with atherosclerosis-related disease using the amplified luminescent proximity homogeneous assay-linked immunosorbent assay. The results of this study demonstrate that serum antibody levels of DIDO1, FOXJ2, and CPSF2 are useful in predicting the onset of atherosclerosis-related acute ischemic stroke caused by kidney failure, hypertension, and diabetes mellitus, respectively.