Perumal J, et al. - In this trial of haptoglobin (Hp) as a diagnostic biomarker for epithelial ovarian cancers (EOCs), researchers used surface-enhanced Raman spectroscopy (SERS)-based in vitro diagnostic assay and quantified Hp in archived ovarian cyst fluids collected from suspicious ovarian cysts and distinguished benign tumors from EOCs. They verified the diagnostic performance of SERS-based assay against the histopathology conclusions and comparisons were made taking into account the results of CA125 test and frozen sections. Malignant samples were found to contain a significantly higher Hp. A sensitivity of 94% and specificity of 91% was observed for Hp measurements using SERS when verified against histology. Area under the curve of 0.966±0.03 was generated, showing the robustness of the method. A sensitivity of 85% and specificity of 90% was observed with CA125 test on the same set of patients. A 100% sensitivity and specificity were seen in frozen section analysis on 65 samples. The SERS-based Hp detection assay demonstrated utility as a promising intraoperative EOC diagnostic test, with a total execution time of <10 minutes and consistent performance seen across different stages of cancer.