Vakamudi K, et al. - Five persons with brain tumor were examined with rsfMRI and tfMRI on a clinical 3T scanner using MultiBand(8)-echo planar imaging with repetition time: 400 ms in order to examine the feasibility of real-time rsfMRI for presurgical mapping of eloquent networks with monitoring of data quality in persons with brain tumors and rsfMRI localization of eloquent cortex im comparison with tfMRI and intraoperative electrocortical stimulation (ECS) in retrospective analysis. Within 1 min of scan time, sensorimotor and language resting-state networks were recognizable. With the exception of discordant rsfMRI and ECS localization of Wernicke area in one person because of potential cortical reorganization and/or changed neurovascular coupling, the Euclidean distance between ECS and rsfMRI connectivity and task-activation in motor cortex, Broca, and Wernicke areas was 5–10 mm. In conclusion, results showed the ability of real-time high-speed rsfMRI for presurgical mapping of eloquent cortex with real-time data quality control, and clinically desirable concordance of rsfMRI with tfMRI and ECS localization.