Chen J, Zheng M, Liu J, et al. - In Chinese patients with nonalcoholic steatohepatitis (NASH), researchers examined bile acid (BA) profile alterations. In patients from two different clinical centers, BA profiles in serum and liver tissues were determined by ultraperformance liquid chromatography coupled to tandem mass spectrometry. In total, 134 candidates were registered to serve as the training (n = 87) and validation (n = 47) cohorts. The ratio of circulating conjugated chenodeoxycholic acids to muricholic acids was elevated from healthy controls to non-NASH people to NASH people in a stepwise manner in the training cohort and was positively linked to the histological NASH severity: steatosis, lobular inflammation, ballooning, and fibrosis stage. Altered BA profile in NASH is closely linked to the severity of liver lesions and has the ability to predict the development of NASH.