Wang Y, Wong GLH, He FP, et al. - Since dual-photon microscopy enables automated quantification of fibrosis-related parameters (q-FPs) and can facilitate large-scale histological studies, researchers validated q-FPs performance in a large histological cohort. Three hundred forty-four individuals with non-alcoholic fatty liver disease (NAFLD) had 428 liver biopsies. For different fibrosis stages, over 25 q-FPs had area under the receiver-operating characteristics curves > 0.90. Changes in the same q-FPs were linked to changes in fibrosis stage in patients with paired liver biopsies. Baseline q-FPs anticipated liver-related events at a median follow-up of 5.6 years. In patients with NAFLD, q-FP is extremely precise in the evaluation of fibrosis. This automated platform can be used as an objective and reliable histological fibrosis assessment in future studies.