Ng KS, et al. – Using paired wholemounts, the authors intended to accurately quantify and neurochemically code the hindgut myenteric plexus, comparing human distal colon and rectum. In human distal colon and rectum, they demonstrated similar neuronal and ganglionic densities and neurochemical profiles. To confirm that there was no regional variation in the human hindgut enteric nervous system (ENS), further investigation of other components of the ENS, incorporating additional immunohistochemical markers were required.