Sonntag B, Eisemann N, Elsner S, et al. - Researchers examined whether the pubertal development and reproductive hormone status during adolescence differ between singletons following ICSI therapy or spontaneous conception (SC). From seven German registration offices (Aachen, Eichstätt, Erfurt, Lübeck, Hamburg, Heidelberg and Schwerin), 274 singleton ICSI-conceived adolescents (141 girls, 133 boys) followed up for the third time, and 273 SC controls (142 girls, 131 boys) were assessed in this prospective controlled study. Tanner staging (breast, genital and pubic hair development), age at menarche and reproductive hormone levels were analyzed in ICSI and SC adolescents at the mean age of 16.5 years for assessing pubertal development. They analyzed differences via multinomial regression (Tanner stages) or t test and linear regression for hormonal assessments. Outcomes revealed largely similar results concerning pubertal development and reproductive hormone levels between ICSI and SC adolescents; except there was a tendency towards lower inhibin B levels as well as significantly higher estradiol levels and a lower testosterone-to-estradiol-ratio in male ICSI adolescents.