Shiraishi T, Shinto E, Nearchou IP, et al. - Researchers tried to find out the prognostic significance of mesothelin (MSLN) expression in different areas of individual colorectal cancers (CRCs) using tissue microarrays (TMAs) by enrolling 314 patients with stage II (T3–T4, N0, M0) CRCs. The TMA blocks were formed Utilizing formalin-fixed paraffin-embedded tissue blocks from patients. They collected tissue core specimens from submucosal invasive front (Fr-sm), subserosal invasive front (Fr-ss), central area (Ce), and rolled edge (Ro) of each tumor. They analyzed the area-specific prognostic significance of MSLN expression. The examination conducted applying area-specific four-point TMAs clearly showed that MSLN expression in stage II CRC was relatively homogeneous within tumors. It was noted that high MSLN expression displayed or tended to show unfavorable prognostic significance regardless of the tumor area.