Gabusi A, Gissi DB, Montebugnoli L, et al. - Ten oral squamous cell carcinoma (OSCC) patients (5 recurrent and 5 nonrecurrent) were examined by the researchers in order to review the influence of intratumoral and intrafield heterogeneity on locoregional control. The mutational analysis reflected that, in evaluating the genetic profiles of tumors, a single tumor sample has limited accuracy. High values of intratumoral heterogeneity (ITH) acknowledging shared mutations between specimens were discovered in both recurrent and non-recurrent OSCC. In contrast, the intrafield genetic heterogeneity was significantly less often in the non-recurrent OSCC group. Heterogeneity within each specimen calculated with variant allele frequency validated that there was better discrimination among recurrent and nonrecurrent groups using nonneoplastic adjacent mucosa than tumor tissue. In arrangement with the theory of field cancerization, intrafield genetic heterogeneity associates with a greater risk of developing loco-regional recurrences and second primaries. In order to decrease the ITH impacts, the analysis of multiple tumor areas should be encouraged.