Prevalence of molecular markers associated with drug resistance of Plasmodium vivax isolates in Western Yunnan Province, China
BMC Infectious Diseases Apr 29, 2020
Wang X, Ruan W, Zhou S, et al. - Given that Plasmodium vivax is the most widely distributed malaria parasite, and its drug resistance poses unique challenges to malaria elimination, researchers here investigated molecular markers linked with drug resistance in the Greater Mekong Subregion (GMS), the global epicenter of multidrug resistance, to facilitate appropriate drug policy in this region. From Yingjiang County, Yunnan Province, along the China–Myanmar border, they obtained dry blood spots from 58 patients out of 109 with P. vivax infection between 2017, December and 2019, March. Per findings, molecular patterns of resistance markers of Pvdhfr, Pvdhps, Pvmdr1 and Pvcrt-o were moderately diverse in imported P. vivax cases to this region. They detected mutations in Pvdhfr at codons 57, 58, 61, 99 and 117 in 27.59%, 48.28%, 27.59%, 32.76% and 48.28% of the isolates, respectively. The most frequent one was single mutant haplotype (I13F57S58T61S99S117I173) (29.31%, 17/58), followed by double mutant haplotype (20.69%, 12/58). Among the three types of tandem repeat variations of Pvdhfr, they most commonly identified deletion type. Mutation genotypes had a lower prevalence of Pvdhps. Single mutant was dominant and accounted for 34.48% (20/58). Pvmdr1 mutations at codons 958 and 1076 were prevalent in 100.00% and 84.48%, respectively. Double and single mutant types constituted 84.48% (49/58) and 15.52% (9/58), respectively. In 11 samples (18.97%, 11/58), K10 “AAG” insertion was identified in chloroquine resistance transporter gene Pvcrt-o.
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