Huisman BW, Burggraaf J, Vahrmeijer AL, et al. - Since there is a requirement for novel instruments for specific real-time detection of occult tumor lesions as well as localization of cancer margins in patients having vulvar squamous cell carcinoma (VSCC), and many tumor-specific imaging techniques are developed to identify malignant tissue by targeting tumor markers, so, researchers investigated, assessed, and summarized possible markers for tumor-specific imaging of VSCC. A systematic cross-database literature search was performed to identify 627 relevant papers. The eligible articles were 22 in total. Experts discovered 7 potential biomarkers (EGFR, CD44v6, GLUT1, MRP1, MUC1, CXCR-4 and VEGF-A). These biomarkers can aid the development of VSCC-specific tracers for real-time and precise localization of tumor tissue prior to, during, and post-treatment. These biomarkers were recognized in a small number of samples, without distinguishing for VSCC-specific hallmarks such as HPV-status.