Bentaleb EM, et al. – This work was planned to report the use of an in house plasmid–based quantitative polymerase chain reaction–high–resolution melting (qPCR–HRM) analysis for the detection of mutations related to rifampicin–resistant Mycobacterium tuberculosis (MTB) in clinical isolates from Moroccan patients. Results demonstrated qPCR–HRM assay as a sensitive, accurate and cost–effective assay for the high–throughput screening of mutation–based drug resistance in tuberculosis (TB) reference laboratories.