Niessen NM, Baines KJ, Simpson JL, et al. - Researchers sought to differentiate as well as quantify macrophages, monocytes and monocyte subsets in induced sputum and blood, and also evaluated their link with inflammatory as well as clinical characteristics of asthma. Using flow cytometry, experts differentiated macrophages, monocytes and subsets in sputum and blood (n = 53; 45 asthma, 8 non‐asthma) and a second asthma sputum cohort (n = 26). Based on surface CD14/CD16, monocyte subsets were detected (CD14⁺⁺CD16^‐ classical, CD14⁺CD16⁺ intermediate and CD14⁺CD16⁺⁺ non‐classical monocytes). According to findings, sputum macrophages, monocytes and subsets were distinguished by applying flow cytometry, and the presence of compartment‐specific dysregulation of monocytes in asthma was revealed. In neutrophilic asthma, sputum samples exhibited a rise in classical and CD206^‐ monocytes, indicating co‐recruitment of monocytes as well as neutrophils to the airways in asthma.