Borys S, et al. - In patients with type 2 diabetes (T2DM) and diabetic foot ulceration (DFU), researchers evaluated the impact of negative pressure wound therapy (NPWT) on gene expression. Using Illumina gene expression arrays and R Bioconductor pipelines based on the limma package, differential gene expression profiling was performed on individuals treated with either NPWT or standard therapy. There were four genes expressed differentially between the two study arms post-treatment, but not pre-treatment, and all four appeared to be involved in wound healing. So initial evidence indicated that the NPWT effect on DFUs might be mediated through differential gene expression. The data presented in this work showed a discovery of the specific molecular mechanisms of NPWT is potentially valuable for its clinical application and development of new therapies.