De Vos A, Van Landuyt L, De Rycke M, et al. - Researchers aimed at determining if and how the clinical outcome is affected by double vitrification and warming of human blastocysts having undergone biopsy once or twice. They conducted a retrospective observational study including 97 cycles of trophectoderm biopsy and preimplantation genetic testing (PGT) on vitrified-warmed embryos followed by a second round of vitrification between March 2015 and December 2019. No biopsy was done on the embryos in 36 warming cycles, before the first vitrification (single biopsy group). In 61 warming cycles, biopsy was performed on the embryos on Day 3 (n = 4) or on Day 5/6 (n = 57) before the first vitrification (double biopsy group). In cycles of failed or inconclusive diagnosis at the first biopsy, a second biopsy was mostly indicated. Outcomes suggest comparable clinical pregnancy rate obtained with double vitrification single biopsy blastocysts vs single vitrification single biopsy blastocysts in the same time period (46%; 2016–2018), however, obtained with double-vitrified double-biopsied blastocysts appeared lower and will require further study.