Ellison PM, et al. - Researchers conducted this prospective, multi-center, cohort study of 562 melanomas treated with Mohs micrographic surgery (MMS) with MART-1 immunostaining in order to recommend excision margins when 100% histologic margin evaluation is not used and to compare real costs of tumor removal with MMS vs standard surgical excision. MMS use in the treatment of melanoma at three academic and eight private practices throughout the US was evaluated. Outcomes revealed that with MART-1, tissue conservation and same-day reconstruction of histologically verified tumor free margins could be safely achieved with Mohs surgery in a convenient, single-day procedure. They recommend using initial surgical margins of at least 10 mm for primary trunk/extremity and 12 mm for head/neck melanomas to achieve histologically negative margins 97% of the time when comprehensive margin evaluation is not used.