Takada K, et al. - Researchers performed a retrospective study to evaluate interobserver agreement among pathologists before and after adding ancillary staining for diagnosing LI and VI in this setting. For this research, 100 specimens of submucosal invasive gastric cancer were included from 100 individuals treated using endoscopic resection. The individual LI and VI statuses were independently assessed by three pathologists (expert, intermediate, and trainee experience levels) by using hematoxylin and eosin (HE)-stained slides, and reevaluated their decisions by analyzing corresponding D2-40-stained and elastin-stained slides. There was an improved interobserver agreement for LI and VI diagnoses, regardless of the pathologist's experience, with both the ancillary D2-40 and elastin staining. The data showed that this ancillary staining may be beneficial in increasing the accuracy of LI and VI diagnoses, advocating to increase the risk stratification of early gastric cancer patients.