Prasad N, Jabbar PK, Jayakumari C, et al. - Considering that late-night salivary cortisol (LNSC) is used as a screening test for Cushing syndrome (CS), but there are no community derived normative data for the normal upper limit in the South Asian population, researchers used commercially available second generation ECLIA to determine the upper limit of normal (97.5^th centile) for late night salivary cortisol in an Asian Indian population. Healthy individuals (aged 18 to 60 years) from 8 Urban and 8 rural clusters of Thiruvananthapuram district have been analyzed. The study involved 30 subjects from an approximate population of 1,000 persons per cluster.Around 2300 hrs to 0000 hrs, saliva samples were obtained and analyzed the next day using Roche COBAS-e-411 and ultrasensitive Cortisol-II kits. Upon removal of inappropriately obtained samples, cortisol values from 474 salivary samples were available for final review. This study establishes for the first time the normal value of late-night salivary cortisol estimated by ECLIA of second generation in healthy community dwelling Asian Indian individuals. In normal Asian Indian population, the salivary cortisol at 2300-0000 hrs is less than 0.25 μg/dl (6.89nmol/l). Menopause causes a significant increase in LNSC, and if not carefully interpreted, can lead to over diagnosis of CS.