Wang Y, et al. - Through this study, a multi-compression approach was used to generate large tissue deformation, and a method to evaluate the 3-D motion of tissue under large deformation was inaugurated. The suggested method first evaluated volume-to-volume incremental displacements via a 3-D region-growing motion-tracking method, provided multiple volumes of ultrasound data, and then, to circumvent error accumulation, possible outliers among all incremental displacements were eliminated. Once large displacement errors had been eliminated, to get accumulated displacements under large tissue deformation, all incremental displacements were registered together. The recommended method was examined with one set of in vivo tumor-bearing ultrasound data obtained from a human individual. A total of 10 small-strain deformation steps were conducted to achieve the ultimate accumulated displacement field, in which the breast lesion and its surrounding were deformed by roughly 6% and 16%, respectively. The contrast-to-noise ratio and signal-to-noise ratio of the elasticity images acquired with the proposed method were all higher in comparison with those received with a 2-D tracking method. Moreover, the breast lesion was certainly evident with a great similarity between the axial strain and B-mode images in three orthogonal views of accumulated axial strain images.